CGSC Strain#: 4303
Strain Designation: P3478      Source of Strain: Cairns & DeLucia
Other Designations: W3110 polA1
Sex: F- Chromosomal Markers: λ^-, e14-, thyA36, IN(rrnD-rrnE)1, polA1(Am), deoC2
Strain Comments:

• Ref. for e14: Van de Putte, 1984, cited below.
• Ref. to the inversion: Hill & Harnish, 1981, cited below.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• thyA36-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• polA1(Am)-- normal levels exonuc.;premature term.of polymerase <1% WT level. MMS^S,UV^S, viable at all temps. Grindley MGG143
• Am = amber(UAG) mutation

References:

• Gross, J., M. Gross 1969. Genetic analysis of an E. coli strain with a mutation affecting DNA polymerase. Nature 224:1166-1168
• DeLucia, P., J. Cairns 1969. Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature 224:1164-1166
• Hill, C.W., B.W. Harnish 1981. Inversions between ribosomal RNA genes of Escherichia coli. Proc.Natl.Acad.Sci.USA 78:7069-7072
• van de Putte, P, R Plasterk, A Kuijpers 1984. A Mu gin complementing function and an invertible DNA region in Escherichia coli K-12 are situated on the genetic element e14. J. Bacteriol. 158(2):517-22.