CGSC Strain#: 5481
Strain Designation: JP2608      Source of Strain: A.J. Pittard
Other Designations: JP2388
Sex: F' Episome/Plasmid: F14-15
   Plasmid Markers/Mutations: trpT179(AS)
Chromosomal Markers: pro-48, tsx-84, λ^-, trpA9605(Am), his-85(Am), trpR55, ilv-632
Strain Comments:

• The amber supprossor supU79 on the episome is now called trpT179. This strain was constructed to replace strain LS446a the original supU79 strain.
• pro-48-- is probably in proB^-.
• trpA9605(Am)-- is an amber mutation. It is suppressed by supD32.
• his-85(Am)-- is an amber mutation. It is suppressed by supD32.
• trpR55-- is trpR2 of Yanofsky. It is a frameshift mutation. Trp levels are indistinguishable from those in trpR deletions or amber mutations (no functional repressor).
• his-85(Am) was formerly called his-29
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• Soll, L, P Berg 1969. Recessive lethals: a new class of nonsense suppressors in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 63(2):392-9.