CGSC Strain#: 673
Strain Designation: Hfr J4      Source of Strain: Jacob & Wollman
Other Designations: P10
Other Designations: Hfr P10
Sex: Hfr    Point of Origin#: 18   Map Position: 91.46   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: thr-1, leuB6(Am), fhuA21, lacY1, glnX44(AS), λ^-, rfbC1, thiE1, malK16(λ^R)
Strain Comments:

• This is a fairly stable Hfr. The insertion is within the malE gene.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• malK16(λ^R)-- F is integrated in the malKgene of the malB region.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant

References:

• Jacob, F., E. Wollman 1957. Analyse de groupes de liaison genetique de differentes souches donatrices d'Escherichia coli. Compt.Rend.Acad.Sci.Paris 245:1840-1843
• Schwartz, M. 1966. Location of the maltose A and B loci on the genetic map of Escherichia coli. J.Bacteriol. 92:1083-1089