CGSC Strain#: 5150
Strain Designation: W945 sdh     
Source of Strain: U. Henning
Sex: F-
Chromosomal Markers: thr-1,
glnX44(AS)
,
galK2(Oc)
,
sdh-1,
λ-,
rpsL265(strR)
,
xylA5Strain Comments: - We don`t think that there is any possibility of tracing the derivation of this strain. Clearly it is not just an sdh- mutant of W945.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Creaghan, IT, JR Guest 1972. Amber mutants of the -ketoglutarate dehydrogenase gene of Escherichia coli K12. J. Gen. Microbiol. 71(2):207-20.