CGSC Strain#: 5380
Strain Designation: LS340      Source of Strain: L. Soll
Sex: F- Chromosomal Markers: λ^-, trpA9605(Am), his-85(Am), IN(rrnD-rrnE)1, metE70, trpR55
Strain Comments:

• This is an ilv⁺, met^- transductant of LS268, described in reference.
• trpA9605(Am)-- is an amber mutation. It is suppressed by supD32.
• his-85(Am)-- is an amber mutation. It is suppressed by supD32.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• trpR55-- is trpR2 of Yanofsky. It is a frameshift mutation. Trp levels are indistinguishable from those in trpR deletions or amber mutations (no functional repressor).
• his-85(Am) was formerly called his-29
• Am = amber(UAG) mutation

References:

• Soll, L, P Berg 1969. Recessive lethals: a new class of nonsense suppressors in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 63(2):392-9.