CGSC Strain#: 7603
Strain Designation: YYC1272      Source of Strain: Cronan & Chang
Sex: F- Chromosomal Markers: Δ(argF-lac)169, λ^-, cfa-5::kan, IN(rrnD-rrnE)1, rph-1, tnaA5
Strain Comments:

• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• cfa-5::kan-- Plasmid pAYW19, Wang et al., 1992, carrying cfa was cut with KpnI (at cfa codon 17 of 382),ligated to 2kbp containing kanR gene of pHP45omega-Km(Fellay et al.1987);used to transform JC7623
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• tnaA5 was formerly called tna-2
• = kanamycin resistant

References:

• Chang, Y.Y., J.E. Cronan 1999. Membrane cyclopropane fatty acid content is a major factor in acid resistance of Escherichia coli. Mol.Microbiol. 33:249-259