CGSC Strain#: 7677
Strain Designation: W3110trpE10220      Source of Strain: C. Yanofsky
Sex: F- Chromosomal Markers: λ^-, trpE10220, IN(rrnD-rrnE)1, rph-1
Strain Comments:

• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)

References:

• Yanofsky, C., J. Ito 1966. Nonsense codons and polarity in the tryptophan operon. J.Mol.Biol. 21:313-334