CGSC Strain#: 7700
Strain Designation: MLK53      Source of Strain: C. Georgopoulos
Sex: F- Chromosomal Markers: λ^-, lpxL10(ts)::mini-Tn10, IN(rrnD-rrnE)1, rph-1
Strain Comments:

• lpxL10(ts)::mini-Tn10-- grows in rich media below 32.5 C, but not at higher temperatures.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = tetracycline resistant
• ts = Temperature Sensitive

References:

• Karow, M., C. Georgopoulos 1991. Sequencing, mutational analysis, and transcriptional regulation of the Escherichia coli htrB gene. Mol.Microbiol. 5:2285-2292
• Karow, M., O. Fayet, A. Cegielska, T. Ziegelhoffer, C. Georgopoulos 1991. Isolation and characterization of the Escherichia coli htrB gene, whose product is essential for bacterial viability above 33 degrees C in rich media. J.Bacteriol. 173:741-750
• Karow, M, S Raina, C Georgopoulos, O Fayet 1991. Complex phenotypes of null mutations in the htr genes, whose products are essential for Escherichia coli growth at elevated temperatures Res. Microbiol. 142(2-3):289-294.