CGSC Strain#: 5536
Strain Designation: PS1537      Source of Strain: Bryant & Sypherd
Sex: F- Chromosomal Markers: lacY1 or lacZ4, glnX44(AS), gal-6, λ^-, rimB2(Cs), rpsL8 or rplL9(L?) or rpsL17, malT1(λ^R), ilvD16, metB1, ΔargH1, thiE1, pro-53
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• rimB2(Cs)-- is a cold-sensitive mutation. The permissive temperature is 37 degrees C, the restrictive temperature is 25 degrees C.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• = strep-resistant
• AS = amber(UAG) suppressor
• Cs = cold-sensitive
• LamR = Lambda-resistant

References:

• Bryant, RE, PS Sypherd 1974. Genetic analysis of cold-sensitive ribosome maturation mutants of Escherichia coli. J. Bacteriol. 117(3):1082-92.