CGSC Strain#: 5688
Strain Designation: JRG596     
Source of Strain: J.R. Guest
Sex: Hfr
Point of Origin#: 1
Map Position: 96.80
Direction of Marker Transfer: Clockwise
Chromosomal Markers: secA214(aziR)
,
Δ(aroP-aceF)73,
fhuA54,
tsx-97,
tonB54,
pps-1,
relA1,
thyA56,
spoT1,
metB1Strain Comments: - This strain is apparently no longer Hfr. This strain was formerly called K DE15.
- thyA56-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- secA214(aziR) was formerly called secA14
- secA214(aziR) was formerly called azi-14
- aziR = azide resistance
References: - Langley, D., J.R. Guest 1977. Biochemical studies of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: isolation and biochemical properties of deletion mutants. J.Gen.Microbiol. 99:263-276