CGSC Strain#: 5690
Strain Designation: NI747      Source of Strain: M. Gellert
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA21, lacY1, glnX44(AS), λ^-, thyA6, malA49, thiE1, deoC1, λ^R, ilv-655
Strain Comments:

• We've received feedback that this strain does not survive in long-term storage in glycerol at -80C.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• λ^R-- Lambda resistant, locus unknown.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• Gellert, M., M.H. O'Dea, A. Itoh, J.-I. Tomizawa 1976. Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase. Proc.Natl.Acad.Sci.USA 73:4474-4478