CGSC Strain#: 5817
Strain Designation: ASH102      Source of Strain: I.B. Holland
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), lacZ43(Fs), lacI22, glnX44(AS), cirA51, thyA117, rpsL228(strR), metB1, creD3
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• thyA117-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• lacI22 was formerly called lacI3
• lacI22 was formerly called lac_i3^-
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Fs = Frameshift mutation
• strR = streptomycin resist.

References:

• Buxton, R.S., I.B. Holland 1973. Genetic studies of tolerance to colicin E2 in Escherichia coli K12. I. Re-location and dominance relationships of cet mutations. Mol.Gen.Genet. 127:69-88