CGSC Strain#: 7690
Strain Designation: CY15682     
Source of Strain: C. Yanofsky
Other Designations: W3110trpR2
Sex: F-
Chromosomal Markers: λ-,
IN(rrnD-rrnE)1,
rph-1,
trpR55Strain Comments: - IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- trpR55-- is trpR2 of Yanofsky. It is a frameshift mutation. Trp levels are indistinguishable from those in trpR deletions or amber mutations (no functional repressor).
References: - Doolittle, W.F., C. Yanofsky 1968. Mutants of Escherichia coli with an altered tryptophanyl-transfer ribonucleic acid synthetase. J.Bacteriol. 95:1283-1294
- Kelley, R.L., C. Yanofsky 1985. Mutational studies with the trp repressor of Escherichia coli support the helix-turn-helix model of repressor recognition. Proc.Natl.Acad.Sci.USA 82:483-487