CGSC Strain#: 14117
Strain Designation: pLC4-14C      Source of Strain:
Sex: F+ Episome/Plasmid: pLC4-14C
   Plasmid Markers/Mutations: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), λ^-, mtl-1, thiE1, galT22, recA56, xylA5, ΔtrpE63, metC , murE , mrdA
   Plasmid Comment: Mixed clone. Hybridizes with Kohara clones 504-506. Carries mrdA at 14.3, metC at 67.9, as well as murE and ftsI at 2.0 minutes. See also pLC4-14A and pLC4-14B.
Chromosomal Markers: thr-1, araC14, leuB6(Am), lacY1, glnX44(AS), galK2(Oc), galT22, λ^-, ΔtrpE63, recA56, xylA5, mtl-1, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• recA56-- Amino acid substitution Arg 60 Cys
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• ΔtrpE63 was formerly called DE(trpE5)
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
• Nishimura, Y., Y. Takeda, A. Nishimura, H. Suzuki, M. Inouye, Y. Hirota 1977. Synthetic ColE1 plasmids carrying genes for cell division in Escherichia coli. Plasmid 1:67-77
• Takeda, Y., A. Nishimura, Y. Nishimura, M. Yamada, S. Yasuda, H. Suzuki, Y. Hirota 1981. Synthetic ColE1 plasmids carrying genes for penicillin-binding protein in Escherichia coli. Plasmid 6:86-98
• Dwivedi, CM, RC Ragin, JR Uren 1982. Cloning, purification, and characterization of beta-cystathionase from Escherichia coli. Biochemistry 21(13):3064-9.
• Neidhardt, F.C., V. Vaughn, T.A. Phillips, P.L. Bloch 1983. Gene-protein index of Escherichia coli K-12. Microbiol.Rev. 47:231-284
• Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151