CGSC Strain#: 7726
Strain Designation: NZN111      Source of Strain: D.P. Clark
Sex: F+ Chromosomal Markers: λ^-, Δpfl-2::Cm, ldhA20::kan, rpoS396(Am), rph-1
Strain Comments:

• pfl ldhA double mutants cannot grow anaerobically on any sugar or sugar alcohol even when supplemented with acetate.
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = kanamycin resistant
• Am = amber(UAG) mutation

References:

• Bunch, P.K., F. Mat-Jan, N. Lee, D.P. Clark 1997. The ldhA gene encoding the fermentative lactate dehydrogenase of Escherichia coli. Microbiology 143:187-195