CGSC Strain#: 6155
Strain Designation: UT205     
Source of Strain: C. Lark
Sex: F-
Chromosomal Markers: trpE68,
thyA42,
rpsL261(strR)
,
metE101,
dnaT11,
deoB20,
arg-69Strain Comments: - This strain is a derivative of E. coli 15 but parts of the 97.5' to 1.5' region could have come from E. coli B or K-12.
- thyA42-- E. coli 15 mutation
- thyA42-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- deoB20-- E. coli 15 mutation.
- deoB20 was formerly called thyR32
- strR = streptomycin resist.
References: - Lark, CA, J Riazi, KG Lark 1978. dnaT, dominant conditional-lethal mutation affecting DNA replication in Escherichia coli. J. Bacteriol. 136(3):1008-17.