CGSC Strain#: 7921
Strain Designation: BW22245      Source of Strain: B.L. Wanner
Sex: F' Episome/Plasmid: F128-Tn10-11
   Plasmid Markers/Mutations: lacIp-4000(lacI^Q), ΔlacZ58(M15), F::Tn10-11, proA , proB , Tn10-11 , lac
Chromosomal Markers: Δ(codB-lacI)3, ΔphoA532, ΔuidA4::pir-116, recA1, rpoS396(Am), rph-1, Δ(phnP-phnD)3330( phnC?)
Strain Comments:

• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• ΔuidA4::pir-116-- Allows plasmids with an R6Kγ origin to replicate to high copy number
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• Am = amber(UAG) mutation
• phnC? = may also delete phnC
• = tetracycline resistant
• M15 = alpha-complemented allele

References:

• Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.