CGSC Strain#: 6700
Strain Designation: JP1111      Source of Strain: Egan & Russell
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: galE45(GalS), λ^-, fabI392(ts), relA1, spoT1, thiE1
Strain Comments:

• galE45(GalS)-- is from strain PL5 of Buttin.
• fabI392(ts)-- is a temperature-sensitive mutation. Addition of 1% NaCl or 12.5% sucrose will reduce temperature sensitivity.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• fabI392(ts) was formerly called envM392
• thiE1 was formerly called thi-1
• GalS = UDPGal accum.toxic if GalKRP+
• ts = Temperature Sensitive

References:

• Egan, A.F., R.R.B. Russell 1973. Conditional mutations affecting the cell envelope of Escherichia coli K-12. Genet.Res. 21:139-152