CGSC Strain#: 6704
Strain Designation: JP5009      Source of Strain: Egan & Russell
Sex: F- Chromosomal Markers: envT406(ts), galE45(GalS), λ^-, relA1, rpsL284(strR), spoT1, thiE1
Strain Comments:

• envT406(ts)-- is a temperature-sensitive mutation, osmotically remedial.
• galE45(GalS)-- is from strain PL5 of Buttin.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1
• GalS = UDPGal accum.toxic if GalKRP+
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Egan, A.F., R.R.B. Russell 1973. Conditional mutations affecting the cell envelope of Escherichia coli K-12. Genet.Res. 21:139-152