CGSC Strain#: 4286
Strain Designation: JRG33      Source of Strain: J.R. Guest
Other Designations: AT1325 lip9
Sex: F- Chromosomal Markers: Δ(gpt-proA)62, lacY1, lipA9, glnX44(AS), galK2(Oc), λ^-, purB15, Rac-0, hisG4(Oc), rfbC1, mgl-51, rpsL35(strR), kdgK51, xylA5, mtl-1, thiE1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Herbert, AA, JR Guest 1968. Biochemical and genetic studies with lysine+methionine mutants of Escherichia coli: lipoic acid and alpha-ketoglutarate dehydrogenase-less mutants. J. Gen. Microbiol. 53(3):363-81.