CGSC Strain#: 7928
Strain Designation: BW18499     
Source of Strain: B.L. Wanner
Sex: F-
Chromosomal Markers: Δ(codB-lacI)3,
λ-,
zfb-223::Tn10,
ackA202,
rpoS396(Am)
,
rph-1,
ΔglnL2001Strain Comments: - Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
- Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
- Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
- zfb-223::Tn10-- : insertion shown by PCR by Nichols et al. 1998 to be in orf b2274 (yfbO) at nucleotide 2,387,022 (51.453 min.)
- zfb-223::Tn10-- cotransduces 18% with purF. The order is zeb-223:: Tn10, purF, aroC
- ackA202-- Strains with ack mutations should be stored frozen (G.F.-L.Ames). This allele was called ackA200 by Feng et al.(1992, see BW18499).
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- Δ(codB-lacI)3 was formerly called Δlac74
- Δ(codB-lacI)3 was formerly called Δ(lac)3
- Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
- Δ(codB-lacI)3 was formerly called X74
- Δ(codB-lacI)3 was formerly called DE(lac)6
- = tetracycline resistant
- Am = amber(UAG) mutation
References: - Feng, J., M.R. Atkinson, W.R. McCleary, J.B. Stock, B.L. Wanner, A.J. Ninfa 1992. Role of phosphorylated metabolic imtermediates in the regulation of glutamine synthetase synthesis in Escherichia coli. J.Bacteriol. 174:6061-6070