CGSC Strain#: 8215
Strain Designation: BW20339/pWM43
Source of Strain: B.L. Wanner
Sex: F'
Episome/Plasmid: pWM43
Plasmid Markers/Mutations: oriR6Kγ,
bla(ApR)
,
tetA,
lacZα,
MCS(SK),
tetR,
orif1,-Episome/Plasmid: F128-Tn10-12
Plasmid Markers/Mutations: lacIp-4000(lacIQ),
ΔlacZ58(M15)
,
F::Tn10-12(kan),
proA ,
proB Chromosomal Markers: Δ(codB-lacI)3,
ΔphoA532,
ΔuidA3::pir+,
recA1,
rpoS396(Am)
,
rph-1,
Δ(phnP-phnD)3330( phnC?)Strain Comments: - Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
- Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
- Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
- ΔuidA3::pir+-- Allows the replication of plasmids with an R6Kγ origin of replication
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- Δ(codB-lacI)3 was formerly called Δlac74
- Δ(codB-lacI)3 was formerly called Δ(lac)3
- Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
- Δ(codB-lacI)3 was formerly called X74
- Δ(codB-lacI)3 was formerly called DE(lac)6
- ΔuidA3::pir+ was formerly called uidA(Δ MluI)::pir+
- Am = amber(UAG) mutation
- phnC? = may also delete phnC
- = tetracycline resistant
- ApR = ampicillin resistant
- = kanamycin resistant
- M15 = alpha-complemented allele
References: - Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.