CGSC Strain#: 5237
Strain Designation: 3.320      Source of Strain: F. Jacob
Other Designations: 3320
Other Designations: CA150
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: lacZ13(Oc), lacI22, λ^-, e14-, relA1, spoT1, thiE1
Strain Comments:

• lacZ13(Oc)-- is lacZ₂ of Jacob, originating in Paris strain 3.320. It is an ochre, polar mutation.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• lacI22 was formerly called lac_i3^-
• lacI22 was formerly called lacI3
• thiE1 was formerly called thi-1
• Oc = ochre(UAA) mutation

References:

• Pardee, A.B., F. Jacob, J. Monod 1959. The genetic control and cytoplasmic expression of "inducibility" in the synthesis of B-galactosidase by E. coli. J.Mol.Biol. 1:165-178