CGSC Strain#: 7042
Strain Designation: CA8445      Source of Strain: J.R. Beckwith
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ^-, e14-, relA1, rpsL136(strR), Δcrp-45, spoT1, ΔcyaA854, thiE1
Strain Comments:

• e14--- : Loss of the e14 element makes the strain McrA^-.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• ΔcyaA854-- is a 200 bp frameshift deletion that generates a new translation start (Glaser et al., below)
• thiE1 was formerly called thi-1
• strR = streptomycin resist.

References:

• Sabourin, D, J Beckwith 1975. Deletion of the Escherichia coli crp gene. J. Bacteriol. 122(1):338-40.