CGSC Strain#: 7061
Strain Designation: DL10      Source of Strain: D.M. LeMaster
Sex: F- Chromosomal Markers: Δ(aroP-aceF)73, galK2(Oc), λ^-, IN(rrnD-rrnE)1, rpsL200(strR), pck-11, ppc-1, maeA1, maeB3, maeR2
Strain Comments:

• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• maeA1-- affects NAD-linked malic enzyme (EC1.1.1.38) and is unmapped.
• maeB3-- affects NADP-linked malic enzyme (EC1.1.1.40)
• maeR2-- is a regulatory mutation for malic enzyme
• galK2(Oc) was formerly called gal₂
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Hansen, EJ, E Juni 1979. Properties of mutants of Escherichia coli lacking malic dehydrogenase and their revertants. J. Biol. Chem. 254(9):3570-5.
• LeMaster, DM, JE Cronan 1982. Biosynthetic production of 13C-labeled amino acids with site-specific enrichment. J. Biol. Chem. 257(3):1224-30.