CGSC Strain#: 4963
Strain Designation: W3110 ColE3, ColI, StrR      Source of Strain: D. Helinski
Sex: F- Episome/Plasmid: ColE3
   Plasmid Markers/Mutations:
Episome/Plasmid: ColI
   Plasmid Markers/Mutations:
   Plasmid Comment: ColI from CA38 (Fredericq)
Chromosomal Markers: λ^-, IN(rrnD-rrnE)1, rpsL149(strR), rph-1
Strain Comments:

• This strain produces the I-type colicin in extremely low amount. ColE3 and ColI came from CA38 (P.Fredericq)
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• strR = streptomycin resist.

References:

• Herschman, H.R., D. Helinski 1967. Comparative study of the events associated with colicin induction. J.Bacteriol. 94:691-699