CGSC Strain#: 4807
Strain Designation: BT1026      Source of Strain: F. Bonhoeffer
Other Designations: AC4807
Other Designations: H1026
Sex: F+ Chromosomal Markers: dnaE1026(ts), tsx-79, thyA55, endA2, rpsL8 or rpsL154(strR), polA1(Am), deo-38, φX^s
Strain Comments:

• dnaE1026(ts)-- has also been called polC.
• thyA55-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• polA1(Am)-- normal levels exonuc.;premature term.of polymerase <1% WT level. MMS^S,UV^S, viable at all temps. Grindley MGG143
• φX^s-- : φX sensitivity is derived from E. coli C
• deo-38 was formerly called thyR34
• = good miniprep dsDNA quality
• Am = amber(UAG) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Wechsler, J.A., V. Nusslein, B. Otto, A. Klein, F. Bonhoeffer, R. Herrmann, L. Gloger, H. Schaller 1973. Isolation and characterization of thermosensitive Escherichia coli mutants defective in deoxyribonucleic acid replication. J.Bacteriol. 113:1381-13