CGSC Strain#: 7731
Strain Designation: ALS216     
Source of Strain: E. Altman
Sex: F-
Episome/Plasmid: F128-xxx
Plasmid Markers/Mutations: Chromosomal Markers: [araD139]B/r,
Δ(argF-lac)169,
λ-,
e14-,
flhD5301,
Δ(fruK-yeiR)725(fruA25),
relA1,
rpsE0(SpcR)
,
rbsR22,
Δ(fimB-fimE)632(::IS1),
deoC1Strain Comments: - [araD139]B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
- Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
- Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
- e14--- : Loss of the e14 element makes the strain McrA-.
- flhD5301-- flagellar defect shown in Komeda and Iino
- Δ(fruK-yeiR)725(fruA25)-- : this fruA25 mutation deletes 29 codons of fruK, fruB and fruBKAp promoter to orf yeiR Peters et al. J.Bact.185:2017.
- Δ(fruK-yeiR)725(fruA25) was formerly called fruA25
- ::IS1 = insertion of IS1
- fruA25 = former designation
- SpcR = spectinomycin resistant
References: - Warren, J., J. Walker, J.R. Roth, E. Altman 2000. Construction and characterization of a highly regulable expression vector pLAC11, and its multipurpose derivatives, pLAC22 and pLAC33. Plasmid 44:138-141