CGSC Strain#: 8077
Strain Designation: AJW1939      Source of Strain: A.J. Wolfe
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), Δ(codB-lacI)3, ackA51::kan, rpsL136(strR), metF159(Am), thiE1
Strain Comments:

• In this background ackA51::kan gives rise to mucoidy on LB at 37 C. In other backgrounds (e.g., MC4100 or MG1655) it does not.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• thiE1 was formerly called thi-1
• = kanamycin resistant
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Kumari, S., C.M. Beatty, D.F. Browning, S.J. Busby, E.J. Simel, G. Hovel-Miner, A.J. Wolfe 2000. Regulation of acetyl coenzyme A synthetase in Escherichia coli. J.Bacteriol. 182:4173-4179
• Wolfe, A.J., J.D. Walker, J.E. Seitz-Partridge, C.F. Lange, B.M. Pruss, M.C. Henk, J.C. Larkin, T. Conway 2003. Evidence that acetyl phosphate functions as a global signal during biofilm development. Mol.Microbiol. 48:977-988