CGSC Strain#: 6548
Strain Designation: M32      Source of Strain: S. Brenner
Sex: F- Chromosomal Markers: galK16, galE15(GalS), λ^-, e14-, relA1, rpsL150(strR), spoT1
Strain Comments:

• galK16-- An IS2 insertion resulting in the truncation of the protein at codon 56
• galE15(GalS)-- is Brenner's gal^-_U38, isolated by UV mutagenesis from his strain CA1.
• galE15(GalS)-- G to A transition resulting in an S122F amino acid substitution
• e14--- : Loss of the e14 element makes the strain McrA^-.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• GalS = UDPGal accum.toxic if GalKRP+
• strR = streptomycin resist.

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