CGSC Strain#: 8046
Strain Designation: EDCM594      Source of Strain: Masters and Merlin
Sex: F- Chromosomal Markers: yafD1::FLKP2(lacZ,kan,Plac), Δlac, λ^-, Δfnr-267, rph-1
Strain Comments:

• Δfnr-267-- was reported for MG1655 by pers.commun.from V.Stewart to B.Bachmann,1986, again to CGSC by V.S.2003. Some MG1655 subcultures and derivatives have it .See also Soupene et al. JBact.185:5611(2003).
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• = kanamycin resistant

References:

• Merlin, C., S. McAteer, M. Masters 2002. Tools for characterization of Escherichia coli genes of unknown function. J.Bacteriol. 184:4573-4581