CGSC Strain#: 6589
Strain Designation: HMS180      Source of Strain: J.W. Campbell
Other Designations: W3110 r^-m⁺
Sex: F- Chromosomal Markers: λ^-, thyA36, IN(rrnD-rrnE)1, rph-1, rpoB331(rifR), hsdR19
Strain Comments:

• thyA36-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• rifR = rifamycin resistance

References:

• Campbell, JL, CC Richardson, FW Studier 1978. Genetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA. Proc. Natl. Acad. Sci. U.S.A. 75(5):2276-80.