CGSC Strain#: 7693
Strain Designation: CY15003     
Source of Strain: C. Yanofsky
Sex: F-
Chromosomal Markers: λ-,
Δ(trpD-trpL)824,
IN(rrnD-rrnE)1,
rph-1,
tnaA5,
trpR55Strain Comments: - IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- trpR55-- is trpR2 of Yanofsky. It is a frameshift mutation. Trp levels are indistinguishable from those in trpR deletions or amber mutations (no functional repressor).
- tnaA5 was formerly called tna-2
References: - Jackson, E.N., C. Yanofsky 1972. Internal deletions in the tryptophan operon of Escherichia coli. J.Mol.Biol. 71:149-161
- Yanofsky, C, V Horn 1981. Rifampin resistance mutations that alter the efficiency of transcription termination at the tryptophan operon attenuator. J. Bacteriol. 145(3):1334-41.