CGSC Strain#: 7744
Strain Designation: A95     
Source of Strain: Russel & Model
Sex: F-
Chromosomal Markers: garB10,
fhuA22,
phoA4(Am)
,
ompF627(T2R)
,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
ilvD132,
trxA11(ts)
,
zif-2223::Tn5,
rrnB-2,
mcrB1,
creC510Strain Comments: - phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- trxA11(ts)-- Pro34 to Ser
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- trxA11(ts) was formerly called fip-1
- zif-2223::Tn5 was formerly called zie::Tn5
- = kanamycin resistant
- Am = amber(UAG) mutation
- Const = constitutive
- T2R = T2 Resistant
- ts = Temperature Sensitive
References: - Russel, M., P. Model 1983. A bacterial gene, fip, required for filamentous bacteriophage F1 assembly. J.Bacteriol. 154:1064-1076
- Russel, M., P. Model 1985. Thioredoxin is required for filamentous phage assembly. Proc.Natl.Acad.Sci.USA 82:29-33