CGSC Strain#: 5782
Strain Designation: JRG26      Source of Strain: J.R. Guest
Other Designations: W1485 lip-2
Sex: F+ Chromosomal Markers: lipA2, glnV42(AS), λ^-, rph-1
Strain Comments:

• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• glnV42(AS) was formerly called supE42
• AS = amber(UAG) suppressor

References:

• Herbert, AA, JR Guest 1968. Biochemical and genetic studies with lysine+methionine mutants of Escherichia coli: lipoic acid and alpha-ketoglutarate dehydrogenase-less mutants. J. Gen. Microbiol. 53(3):363-81.