CGSC Strain#: 8183
Strain Designation: BW31003     
Source of Strain: B.L. Wanner
Sex: F-
Chromosomal Markers: Δ(araD-araB)567,
ΔlacZ4787(::rrnB-3)
,
λ-,
att80(CmR)
::KZ28araBp-GFP,
Δ(rhaD-rhaB)568,
hsdR514Strain Comments: - Insertion of pKZ28 at attφ80 site, resulting in GFP on the chromosome, inducible by arabinose. Also CmR from pKZ28 is present.
- Δ(araD-araB)567-- This deletion extends from ~25 bp upstream of the araB start codon to ~8 bp into the beginning of the araD gene
- ΔlacZ4787(::rrnB-3)-- : 4 tandem copies of the rrnB transcriptional terminator inserted by gene replacement into the region extending from near the SacII site near the N-terminus of lacZ through the promoter.
- Δ(araD-araB)567 was formerly called Δ araBADAH33
- Δ(rhaD-rhaB)568 was formerly called Δ rhaBADLD78
- ΔlacZ4787(::rrnB-3) was formerly called ::rrnB-4
- ΔlacZ4787(::rrnB-3) was formerly called Δ lacZWJ16
- ::rrnB-3 = 4 copies of rrnB inserted
- CmR = chloramphenicol resistant
References: - Zhou, L., K. Zhang, B.L. Wanner 2004. Chromosomal expression of foreign and native genes from regulatable promoters in Escherichia coli. Methods Mol Biol 267:123-134