CGSC Strain#: 6989
Strain Designation: ED3867      Source of Strain: J. Maule
Sex: F- Chromosomal Markers: Δ(codB-lacI)3, tsx-80, galK48, λ^def, trp-52, lys-30, rpsL160(strR), malA37
Strain Comments:

• This is an F- derivative of strain M1174 of Beutin and Achtman. Δ 3 is from Hfr 3000 X74.
• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• strR = streptomycin resist.

References:

• Beutin, L., M. Achtman 1979. Two Escherichia coli chromosomal cistrons, sfrA and sfrB, which are needed for expression of F factor tra functions. J.Bacteriol. 139:730-737