CGSC Strain#: 8203
Strain Designation: WTZ1011 ColE2      Source of Strain: J.E. Wertz
Sex: F- Episome/Plasmid: ColE2-P9
   Plasmid Markers/Mutations:
Chromosomal Markers: λ^-, gyrA586(NalR), IN(rrnD-rrnE)1, rpsL-(strR), rph-1
Strain Comments:

• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• NalR = Nalidix.acidResist.
• strR = streptomycin resist.

References:

• Mock, M, M Schwartz 1978. Mechanism of colicin E3 production in strains harboring wild-type or mutant plasmids. J. Bacteriol. 136(2):700-7.