CGSC Strain#: 6996
Strain Designation: 1100-2     
Source of Strain: P.M. Rabinovich
Other Designations: BB2
Other Designations: BBM2
Other Designations: BSV1100-2
Sex: F-
Chromosomal Markers: glnX44(AS)
,
λ-,
mcrA0,
rfbC1,
endA1,
spoT1,
thiE1,
mcrB9999,
hsdR29Strain Comments: - This strain is thought to carry a mutation number which renders it permeable to riboflavin.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- endA1-- G to A transition mutation resulting in E208K amino acid substitution.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- = good miniprep dsDNA quality
- AS = amber(UAG) suppressor
References: - Bandrin, S.V., P.M. Rabinovich, A.I. Stepanov 1983. [Three linkage groups of genes involved in riboflavin biosynthesis in Escherichia coli]. Genetika(Sov.Genet.) 19:1419-1425
- Bandrin, SV, PM Rabinovich, AI Stepanov 1983. [3 linkage groups of the genes of riboflavin biosynthesis in Escherichia coli]. Genetika 19(9):1419-25.