CGSC Strain#: 8266
Strain Designation: BW18812      Source of Strain: B.L. Wanner
Sex: F- Chromosomal Markers: Δ(codB-lacI)3, ΔphoA532, ΔuidA5, [phn_B]
Strain Comments:

• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• ΔuidA5-- has 695 bp deleted between MluI sites
• [phn_B]-- functional phn locus from E. coli B
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6

References:

• Metcalf, W.W., B.L. Wanner 1993. Construction of new beta-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement. Gene 129:17-25