CGSC Strain#: 7800
Strain Designation: KS476     
Source of Strain: K.L. Strauch
Other Designations: K1343
Sex: F-
Chromosomal Markers: ΔdegP41::kan,
Δ(codB-lacI)3,
galK0,
galE9999(GalS)
,
lpp-5508,
rpsL-(strR),
thi-Strain Comments: - ΔdegP41::kan-- The PstI fragment was removed from degP and replaced with the KanR cassette from pUCK-4
- Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
- Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
- Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
- lpp-5508-- results in outer membrane that is leaky to macromolecules
- Δ(codB-lacI)3 was formerly called Δlac74
- Δ(codB-lacI)3 was formerly called Δ(lac)3
- Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
- Δ(codB-lacI)3 was formerly called X74
- Δ(codB-lacI)3 was formerly called DE(lac)6
- ΔdegP41::kan was formerly called degP41(Δ(PstI))::kan
- = kanamycin resistant
- GalS = UDPGal accum.toxic if GalKRP+
- strR = streptomycin resist.
References: - Strauch, K.L., J.R. Beckwith 1988. An Escherichia coli mutation preventing degradation of abnormal periplasmic proteins. Proc.Natl.Acad.Sci.USA 85:1576-1580
- Strauch, K.L., K.R. Johnson, J.R. Beckwith 1989. Characterization of degP, a gene required for proteolysis in the cell envelope and essential for growth of Escherichia coli at high temperature. J.Bacteriol. 171:2689-2696