CGSC Strain#: 4239
Strain Designation: JC12     
Source of Strain: A.J. Clark
Sex: Hfr
Point of Origin#: 12
Map Position: 68.80
Direction of Marker Transfer: Clockwise
Chromosomal Markers: fhuA2::IS2,
lacY1 or lacZ4,
tsx-1,
glnX44(AS)
,
gal-6,
λ-,
rfbC1,
purF1,
xyl-7,
mtlA2,
metB1Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- purF1-- is a very stable mutation and may be an intragenic deletion.
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- xyl-7 was formerly called xylA7
- ::IS2 = Insertion of IS2
- AS = amber(UAG) suppressor
References: - Clark, A.J. 1963. Genetic analysis of a "double male" strain of Escherichia coli K-12. Genetics 48:105-120
- Clark, A.J., W.K. Maas, B. Low 1969. Production of a merodiploid strain from a double male strain of E. coli K12. Mol.Gen.Genet. 105:1-15