CGSC Strain#: 6515
Strain Designation: LCK8      Source of Strain: L. Comai
Sex: F- Chromosomal Markers: lacY1 or Δ(cod-lacI)6, glnX44(AS), galK2(Oc), galT22, λ^-, metB1, hsdR2, opgB202::Tn10
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• opgB202::Tn10-- cotransduces 34% with serB and 13% with thr and ca. 50% with hsd. Shown by PCR analysis (Nichols et al.) to be within opgB at nucleotide 4,595,002 (99.0 min.).
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• opgB202::Tn10 was formerly called
• = tetracycline resistant
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References: