CGSC Strain#: 6206
Strain Designation: KL385     
Source of Strain: M.C. Cappello
Sex: F-
Chromosomal Markers: araC14,
leuB6(Am)
,
lacZ118(Oc)
,
lacI22,
λ-,
serU132(AS)
,
gyrA49(NalR),
srl-1,
alaS5(ts)
,
argA21,
rpsL109(strR)
,
xylA5,
mtl-1,
metE70Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- lacZ118(Oc)-- is the lacZ allele originating in Paris strain Hfr 3000 U118. It is an ochre mutation.
- lacI22 was formerly called laci3-
- lacI22 was formerly called lacI3
- serU132(AS) was formerly called supD32
- serU132(AS) was formerly called Su1+
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- NalR = Nalidix.acidResist.
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Theall, G, KB Low, D Soll 1977. Suppression of a defective alanyl-tRNA synthetase in Escherichia coli: a compensatory mutation to high alanine affinity. Mol. Gen. Genet. 156(2):221-7.