CGSC Strain#: 6342
Strain Designation: DM936     
Source of Strain: D.W. Mount
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
Δ(gpt-proA)62,
lacY1,
tsx-33,
glnX44(AS)
,
galK2(Oc)
,
λ-,
Rac-0,
recA1,
rpsL31(strR)
,
kdgK51,
xylA5,
mtl-1,
argE3(Oc)
,
lexA41(ts)
,
lexA3(Ind-)
Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- lexA41(ts)-- was formerly called tsl-1.
- lexA3(Ind-)-- : Non-cleavable LexA repressor protein; defective in SOS induction, UV sensitive
- lexA3(Ind-)-- This mutation results in a G85D amino acid substitution at the lexA cleavage site.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Ind- = non-inducible
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Mount, DW, AC Walker, C Kosel 1975. Effect of tsl mutations in decreasing radiation sensitivity of a recA- strain of Escherichia coli K-12. J. Bacteriol. 121(3):1203-7.