CGSC Strain#: 6419
Strain Designation: SK2210      Source of Strain: S.R. Kushner
Sex: F- Chromosomal Markers: Δ(lacY-lacZ)286, tsx-3, glnX44(AS), λ^-, lacZ95=phi80dIIlacZ9, hisG4(Oc), rpsL281(strR), xyl-7, mtl-1, rbsD296::Tn10, ΔargH1
Strain Comments:

• Δ(lacY-lacZ)286-- is a deletion of part of lacY and part of lacZ which has been called lacMS286.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• lacZ95=phi80dIIlacZ9-- is an intragenic deletion of lacZ which does not overlap DE286, carried on φ80. (Konrad, 1977, J.Bacteriol.130:167)
• rbsD296::Tn10-- cotransduces 68% with ilv, counterclockwise. Shown by PCR analysis to be within the rbsD gene at nucleotide 3,931,475 (84.744 min.)
• Δ(lacY-lacZ)286 was formerly called lacMS286
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• lacZ95=phi80dIIlacZ9 was formerly called lacBK1
• rbsD296::Tn10 was formerly called zie-296::Tn10
• xyl-7 was formerly called xylA7
• = tetracycline resistant
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

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