CGSC Strain#: 5923
Strain Designation: JW165      Source of Strain: J.A. Wechsler
Sex: F- Chromosomal Markers: lacZ53(Am), λ^-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), rha-5, malB45, deoC2
Strain Comments:

• The inversion between rrnD and rrnE comes from strain W3110.
• lacZ53(Am)-- is the Paris lac^-_{Y14 amber}.
• thyA36-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Sclafani, R.A., J.A. Wechsler 1981. High frequency of genetic duplications in the dnaB region of the Escherichia coli K12 chromosome. Genetics 98:677-689