CGSC Strain#: 5102
Strain Designation: HF4714      Source of Strain: P. Howard-Flanders
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, glnX44(AS), galK2(Oc), λ^-, hisG4(Oc), rpsL31(strR) or rpsL154(strR), argE3(Oc) or arg-49
Strain Comments:

• This is an E. coli K-12 x E. coli C hybrid. It is φX sensitive and at least Thy⁺, Rec⁺ and Mtl⁺ Xyl⁺ are also derived from E. coli C..
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

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