CGSC Strain#: 5104
Strain Designation: PA200 S
RF
1      Source of Strain: F. Jacob
Sex: F' Episome/Plasmid: F100
Plasmid Markers/Mutations:
Plasmid Comment: This is the long F-gal, F1-gal
Chromosomal Markers: thr-1, leuB6(Am), fhuA2::IS2, lacY1, glnX44(AS), gal-6, λ-, hisG1, malT1(λR), xyl-7, mtlA2, ΔargH1, rplL9(L?), thiE1
Strain Comments: - F100 is the long F-gal; F1-gal.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- L? = assignment uncertain
- LamR = Lambda-resistant
References: - Brenner, S., J.R. Beckwith 1965. Ochre mutants, a new class of suppressible nonsense mutants. J.Mol.Biol. 13:629-637
- Ohtsubo, E, MT Hsu 1978. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB. J. Bacteriol. 134(3):778-94.