Mutation idnR735(del)::kan
- Name: idnR735(del)::kan
- Type: Insertion
- Mutation of Gene: idnR
- Approx. Map Location: 96.73
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GAGAATACGTGTAATGAAGAGAAAATAACCTAAAGCGTGTTGCCGTGATAATTCCGGGGATCCGTCGACC and GTAGTTTCGGGGGAAGATTGAAGATTCCCCCGACGTTGGGAGAAGTTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW4221-5 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, ΔidnR735::kan, hsdR514